AQA · Chemistry · 8462
GCSE Chemistry: Chromatography
Chemical analysis · AQA GCSE Chemistry (8462), specification point 4.8.1.3. Cards are generated from the exam board's own specification for "Chromatography", and every card links back to the page it came from. Specification © AQA — used with attribution.
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What are the two phases involved in chromatography?
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A stationary phase and a mobile phase.
What does separation in chromatography depend on?
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The distribution of substances between the stationary phase and the mobile phase.
What is the formula for calculating an Rf value?
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Rf = distance moved by substance ÷ distance moved by solvent.
From where to where is the distance moved by a compound measured when finding its Rf value?
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From the origin (baseline) to the centre of the spot.
In paper chromatography, the ? phase is the solvent that moves up the paper.
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mobile
Why can Rf values help identify a compound?
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Different compounds have different Rf values in a given solvent, so a measured Rf can be matched against known values.
How does a chromatogram show that a substance is pure?
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A pure compound produces a single spot in all solvents.
How does a chromatogram show that a substance is a mixture?
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The mixture separates into more than one spot (depending on the solvent used).
A pure compound produces a ? spot on a chromatogram in all solvents.
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single
What is the maximum possible Rf value, and why?
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1 — a substance cannot travel further than the solvent front that carries it.
What does AQA GCSE Chemistry Required Practical 6 investigate?
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Using paper chromatography to separate and distinguish coloured substances, including calculating Rf values.
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